A Precision Lab Incubator Equipment from china Laboratory Incubator Manufacturer is an important piece of equipment in any laboratory, but reliable results require the proper use and maintenance of your equipment. What's more, some models can get quite expensive, and you may not want to replace your machine as often.
1. Position your device correctly Proper placement of your equipment ensures that it operates efficiently and minimizes exposure to potential contaminants. When choosing the best location for your laboratory incubator, you need to consider the following factors:
Doors and vents can blow in pollutants and increase the chance of fungal growth. What's more, they create airflow that affects the temperature stability of the device. Direct sunlight can cause temperature fluctuations and problems with the anti-condensation feature. You need enough space (at least three inches) around the device so that heat can escape and power cords and outlets are easily accessible. Place floor-standing incubators on shelves to reduce the risk of influx of contaminants when the door is open. Avoid damp, damp areas where fungus can grow. Place the device away from sources of vibration, such as shakers, blenders, or refrigerators, as vibration can affect cell growth. Make sure the area around the device is as clean as possible. The ideal conditions for a laboratory incubator are a temperature-controlled environment and clean room conditions. Since this is usually neither practical nor feasible, considering the above factors is sufficient for most applications.
2. Monitor the temperature The thchamber heating incubator is typically maintained at 37°C for optimal growth of cell cultures. Temperature deviations can inhibit growth or even destroy cultures. A temperature sensor is included in the unit, but how do you know you can always rely on your sensor? One way to ensure the correct temperature is to use a second thermometer.
If your incubator has a glass door, you can install a calibrated thermometer inside the glass and read it without opening the door. You can check this against the sensor temperature and if they are different you know the sensor needs to be recalibrated.
Avoiding opening and closing doors unnecessarily helps keep the temperature stable (and prevents contaminants from entering the chamber). If you are concerned about the incubator door being opened unintentionally, you can choose a unit with a door lock.
3. Monitor humidity and carbon dioxide Optimal growth conditions for cell cultures also include specific humidity levels and, in some cases, CO2 levels. Tissues and cells are sensitive to changes in these parameters and therefore require close monitoring.
If the humidity is too low, your cell culture medium may evaporate, or your growth medium may become too concentrated. The ideal humidity is usually around 95%, to keep it at this level you need to ensure that the water pan (under the incubator) is never at risk of drying out.
For CO2 incubators, you need to monitor CO2 levels. This is usually kept at 5% to maintain a constant pH for optimal growth. The CO2 sensor helps by indicating when and how much CO2 needs to be added to the chamber. You can check CO2 levels every few months with an external gas analyzer.
4. Regular temperature calibration We discussed monitoring temperature above, but thermometers should also be regularly verified and calibrated. The exact timeline will depend on your application, but each month is a good guide. The unit should also be recalibrated if verification determines that the unit is required and after an abnormal event such as a power outage or spill cleanup.
For reproducible results, calibration should be performed at normal operating temperatures. For incubators used in critical or sensitive experiments, you should also consider arranging for annual calibration by an external facility.
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